The Effect of Echinacea Purpurea L. (Eastern Purple Coneflower) Essential Oil on Hematological Parameters and Gut Microbial Population of Zebrafish (Danio Rerio) With Aflatoxicosis

Background: Aflatoxin is one of the most important fungal toxins with documented hepatotoxic, teratogenic, and immunosuppressive properties. This mycotoxin mainly produced by species genus Aspergillus in feed. Therefore, application compounds, which prevent complications aflatoxins without losing feed quality, highly beneficial. Objectives: study aimed to determine effect Echinacea purpurea L. essential oils on regulation microbial population gastrointestinal tract some blood factors aflatoxin-fed zebrafish. Methods: Zebrafish were divided into four groups 45 fish three replicates: control (G1); G2, fed containing 500 µg/kg E. oils; G3, oil 3 ppm aflatoxin B1(AFB1); G4, AFB1. The diets for 60 days. After this period, they euthanized, was collected from tail vein, smears prepared. Fish hepatopancreas used measure alanine aminotransferase, aspartate transaminase, alkaline phosphatase enzymes an auto-analyzer. Also, intestinal contents cultured evaluate population. Results: Results showed that liver increased group (P<0.05), concurrent use along AFB1 reduced compared AFB1-treated group. Moreover, could convert pathogens. Differential counts G2 G3 increase percentages neutrophils thrombocytes. Conclusion: According results study, reduce adverse effects chronic contamination Nevertheless, more studies are needed better understand immunological function zebrafish its mechanism action against